LB medium = Kolibarkteria (at lesa um á s. 165 a.b. í lívfrøðibókini)
LB agar evni (gele) ella vanliga nevnt blóð agar
Kolibakteriur eru følsomar fyri antibiotika
1 ílat : LB medium (uttan antibiotika) + kolibakteria
2 ílat : LB medium (v/antibiotika) + prøvi frá 1 ílat
Fyri at tynna bakteriurnar frá einari koloni í LB medium, ger man tær til einkultar bakteriur.
Bakteriur kunna gott liva av LB medium, tí tað verður nýtt sum føðsluevni (næringsstof).
Úrslit:
Antibiotika drap bakteriurnar í LB medium, men í tí LB medium har einki antibiotika varð í, vuksu koloniurnar og meira bakteriur komu burtur úr. Hatta hendi, av tí at LB medium er eitt føðslievni fyri bakteriurnar, men tá antibiotika kemur í, verða tær allar dripnar.
Agar:
An agar plate is a sterile Petri dish that contains a growth medium (typically agar plus nutrients) used to culture microorganisms or small plants like the moss Physcomitrella patens.
Selective growth compounds may also be added to the media, such as antibiotics.
Individual microorganisms placed on the plate will grow into individual colonies, each a clone genetically identical to the individual ancestor organism (except for the low, unavoidable rate of mutation). Thus, the plate can be used either to estimate the concentration of organisms in a liquid culture or a suitable dilution of that culture using a colony counter, or to generate genetically pure cultures from a mixed culture of genetically different organisms, using a technique known as "streaking". In this technique, a drop of the culture on the end of a thin, sterile loop of wire, sometimes known as an inoculator, is streaked across the surface of the agar leaving organisms behind, a higher number at the beginning of the streak and a lower number at the end. At some point during a successful "streak", the number of organisms deposited will be such that distinct individual colonies will grow in that area which may be removed for further culturing, using another sterile loop.
Det er gratis at oprette en konto